UEFA on Monday opened an investigation into Cristiano Ronaldo's goal celebration after scoring a hat-trick in Juventus' Champions League comeback win against Atletico Madrid last week.

European football's governing body said Ronaldo was facing charges of "improper conduct" after he mimicked Diego Simeone's crotch-grabbing 'cojones' gesture in last Tuesday's 3-0 win that sent Juventus through 3-2 on aggregate.

After scoring the decisive penalty four minutes from time, Ronaldo turned towards the Atletico fans and replicated Simeone's gesture after the Spaniards' 2-0 first leg win by grasping his genitals and thrusting out his groin.

Juventus take on Dutch club Ajax in the Champions League quarter-finals over two legs, on April 10 in Amsterdam and April 16 in Turin.

Five-time Champions League winner Ronaldo is the record goalscorer in the European competition with 124 goals in 160 matches.

Coach Max Allegri has said he is confident Ronaldo will not be fined or suspended.

"I think everyone celebrated their own way on the field and in the stands. I saw nothing strange, just a celebration. There won't be a ban," said Allegri.

Simeone was fined 20,000 euros by UEFA for his gesture.

"He will have seen how I did it at the Wanda Metropolitano and, like me, was trying to show his character," said the Argentinian coach.

A decision will be taken by UEFA on March 21.